Nawet po roku od leczenia- obecne żywe krętki
Po roku leczenia test w kierunku Borrelia burgdorferi był pozytywny, oraz z tkanek można było wyhodować bakterie.Czy da się ta ,,piekielna,, chorobę wyleczyć ?
Antybiotyk podawano myszom przez 30 dni.! - ceftriakson.Nie bylo badan na ludziach ale wynika z tego ze ceftrakson nie zabija wszystkich form Borreli.
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Mice were administered 16 mg/kg ceftriaxone in 500 µl 0.9% normal saline intraperitoneally twice daily for 5 days and then once daily for 25 days. This dosage regimen was utilized as a model for induction and analysis of persisting non-cultivable B. burgdorferi, since it has been previously shown to result in persistence of non-cultivable B. burgdorferi when administered at 3–4 weeks or 3–4 months of B. burgdorferi N40 infection, and C3H mice were subsequently tested at 1 to 3 months after completion of treatment [14], [15], [16], [17]. Previous studies in this laboratory have shown that the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of ceftriaxone is 0.015 µg/ml and 0.06 µg/ml, respectively. Serum ceftriaxone levels were 93 µg/ml, 20 µg/ml, and 2 µg/ml at 1, 2 and 4 hours, respectively, and undetectable at 8 hours in C3H mice following a single injection of 16 mg/kg ceftriaxone, using an agar-based Staphylococcus aureus inhibition assay [17]. Thus, peak serum ceftriaxone levels exceeded the B. burgdorferi cN40 MIC and MBC [14], [15], [16], [33].
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http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0086907
Po roku leczenia test w kierunku Borrelia burgdorferi był pozytywny, oraz z tkanek można było wyhodować bakterie.Czy da się ta ,,piekielna,, chorobę wyleczyć ?
Antybiotyk podawano myszom przez 30 dni.! - ceftriakson.Nie bylo badan na ludziach ale wynika z tego ze ceftrakson nie zabija wszystkich form Borreli.
Abstract
The agent of Lyme borreliosis, Borrelia burgdorferi, evades host immunity and establishes persistent infections in its varied mammalian hosts. This persistent biology may pose challenges to effective antibiotic treatment. Experimental studies in dogs, mice, and non-human primates have found persistence of B. burgdorferi DNA following treatment with a variety of antibiotics, but persisting spirochetes are non-cultivable. Persistence of B. burgdorferi DNA has been documented in humans following treatment, but the significance remains unknown. The present study utilized a ceftriaxone treatment regimen in the C3H mouse model that resulted in persistence of non-cultivable B. burgdorferi in order to determine their long-term fate, and to examine their effects on the host. Results confirmed previous studies, in which B. burgdorferi could not be cultured from tissues, but low copy numbers of B. burgdorferi flaB DNA were detectable in tissues at 2, 4 and 8 months after completion of treatment, and the rate of PCR-positive tissues appeared to progressively decline over time. However, there was resurgence of spirochete flaB DNA in multiple tissues at 12 months, with flaB DNA copy levels nearly equivalent to those found in saline-treated mice. Despite the continued non-cultivable state, RNA transcription of multiple B. burgdorferi genes was detected in host tissues, flaB DNA was acquired by xenodiagnostic ticks, and spirochetal forms could be visualized within ticks and mouse tissues by immunofluorescence and immunohistochemistry, respectively. A number of host cytokines were up- or down-regulated in tissues of both saline- and antibiotic-treated mice in the absence of histopathology, indicating host response to the presence of non-cultivable, despite the lack of inflammation in tissues.---------------------------------------
Mice were administered 16 mg/kg ceftriaxone in 500 µl 0.9% normal saline intraperitoneally twice daily for 5 days and then once daily for 25 days. This dosage regimen was utilized as a model for induction and analysis of persisting non-cultivable B. burgdorferi, since it has been previously shown to result in persistence of non-cultivable B. burgdorferi when administered at 3–4 weeks or 3–4 months of B. burgdorferi N40 infection, and C3H mice were subsequently tested at 1 to 3 months after completion of treatment [14], [15], [16], [17]. Previous studies in this laboratory have shown that the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of ceftriaxone is 0.015 µg/ml and 0.06 µg/ml, respectively. Serum ceftriaxone levels were 93 µg/ml, 20 µg/ml, and 2 µg/ml at 1, 2 and 4 hours, respectively, and undetectable at 8 hours in C3H mice following a single injection of 16 mg/kg ceftriaxone, using an agar-based Staphylococcus aureus inhibition assay [17]. Thus, peak serum ceftriaxone levels exceeded the B. burgdorferi cN40 MIC and MBC [14], [15], [16], [33].
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http://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0086907
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