Naukowcy zaobserwowali uaktywnianie się zestawu genów w komórkach krwi w momencie zakażenia

Naukowcy Uniwersytetu Kalifornijskiego i Uniwersytetu Johna Hopkinsa w USA na łamach pisma "mBio" omówili nową procedurę, która pozwala wykryć infekcję krótko po tym, jak krętki przenoszone przez kleszcza, wnikną do organizmu, nawet wtedy, gdy dotychczasowej testy wskazują mylący wynik negatywny.

Naukowcy zaobserwowali uaktywnianie się zestawu genów w komórkach krwi w momencie zakażenia.

Pomoze to lekarzom skuteczniej wykrywac Borelioze i szybko zaczynać leczenie.

Longitudinal Transcriptome Analysis Reveals a Sustained Differential Gene Expression Signature in Patients Treated for Acute Lyme Disease.

RESULTS

Patient enrollment, sample collection, and transcriptome analysis. This study included a cohort of 29 patients with acute Lyme disease and 13 matched controls without acute illness. Transcriptome profiling by RNA sequencing (RNA-Seq) and pathway analysis were performed with PBMC samples collected at three time points, V1 (time of acute Lyme disease diagnosis and prior to starting antibiotic therapy), V2 (immediately after the completion of a 3-week course of doxycycline treatment), and V5 (6 months

after the completion of therapy) (Fig. 1). Approximately 73 ( 43[standard deviation]) million reads were generated per sample,and on average, 64.9% of the genes had nonzero counts (see Fig.S1 in the supplemental material).....

http://sciencemission.com/data/attachment.php?id=2356&for_session=5ad3e0b04a991965d8bfc7edc8e058d3.

ABSTRACT

Lyme disease is a tick-borne illness caused by the bacterium Borrelia burgdorferi, and approximately 10 to 20% of patients report persistent symptoms lasting months to years despite appropriate treatment with antibiotics. To gain insights into the molecular basis of acute Lyme disease and the ensuing development of post-treatment symptoms, we conducted a longitudinal transcriptome study of 29 Lyme disease patients (and 13 matched controls) enrolled at the time of diagnosis and followed for up to 6 months. The differential gene expression signature of Lyme disease following the acute phase of infection persisted for at least 3 weeks and had fewer than 44% differentially expressed genes (DEGs) in common with other infectious or noninfectious syndromes. Early Lyme disease prior to antibiotic therapy was characterized by marked upregulation of Toll-like receptor signaling but lack of activation of the inflammatory T-cell apoptotic and B-cell developmental pathways seen in other acute infectious syndromes. Six months after completion of therapy, Lyme disease patients were found to have 31 to 60% of their pathways in common with three different immune-mediated chronic diseases. No differential gene expression signature was observed between Lyme disease patients with resolved illness to those with persistent symptoms at 6 months post-treatment. The identification of a sustained differential gene expression signature in Lyme disease suggests that a panel of selected human host-based biomarkers may address the need for sensitive clinical diagnostics during the “window period” of infection prior to the appearance of a detectable antibody response and may also inform the development of new therapeutic targets.

http://mbio.asm.org/content/7/1/e00100-16


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Researchers at UC San Francisco and Johns Hopkins may have found a new way to diagnose Lyme disease, based on a distinctive gene "signature" they discovered in white blood cells of patients infected with the tick-borne bacteria.

https://www.sciencedaily.com/releases/2016/02/160212093702.htm

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